RNA Preparation and Purification
New England Biolabs, Inc. NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) – 24 reactions
The NEBNext Globin & rRNA Depletion Kit (Human/Mouse/Rat) employs the NEBNext RNase H-based RNA depletion workflow to deplete adult, embryonic and fetal globin mRNA (HBA1/2, HBB, HBD, HBM, HBG1/2, HBE1, HBQ1 and HBZ), cytoplasmic rRNA (5S, 5.8S, 18S, 28S, ITS and ETS) and mitochondrial rRNA (12S and 16S). The kit is effective with human, mouse and rat total RNA preparations, both intact and degraded. The resulting depleted RNA is suitable for RNA-seq, random-primed cDNA synthesis, or other downstream RNA analysis. This kit can also be used following poly(A) mRNA enrichment (e.g., using the NEBNext poly(A) mRNA Magnetic Isolation Module NEB #E7490), so that the final depleted RNA contains only mRNA of interest and no non-coding RNA.
New England Biolabs, Inc. NEBNext Low-bias Small RNA Library Prep Kit - 24 reactions
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 ng - 1,000 ng) or enriched small (0.05 ng - 5 ng) RNA. The kit's reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries. This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample. For optimal flexibility in multiplexing, the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5), which must be purchased separately; 480 indices are available.
- Generates libraries with the lowest bias among commercially available kits
- Fastest workflow (~3.5 hours) for single-day libraries
- Multiplexable with up to 480 UDIs
- Simplified, gel-free workflow
- 18-month shelf life
Omega Biotek Inc E.Z.N.A. HP Total RNA Kit, 200 preps, RNA binding capacity 100 μg (RNA >200 bases), silica-based spin column
The OMEGA BIOTEK E.Z.N.A. HP Total RNA Kit provides an efficient solution for the isolation of high-quality total RNA from eukaryotic cells and tissues. It employs a streamlined purification process, integrating sample homogenization and genomic DNA removal into a single step, utilizing advanced column technology. This kit enhances user safety by eliminating toxic organic solvents and simplifies the protocol for RNA purification, making it suitable for various sensitive applications.
- Rapid isolation in approximately 25 minutes for quick experimental turnaround
- High-performance RNA quality and yield
- Versatile compatibility with various cell and tissue types
- Safe and convenient with no toxic organic solvents
- Optimized for sensitive downstream applications like RT-PCR and next-generation sequencing
- High binding capacity of 100 μg for RNA >200 bases
Zymo Research Corporation Quick-RNA MagBead (4x 96 preps)
Total RNA is eluted into 50 l of DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT/PCR, hybridization, etc.
MP Biomedicals™ FastRNA™ Win Kit
For easy and quick isolation of total RNA from a wide variety of plant samples
Fisher BioReagents™ SurePrep™ RNA/Protein Purification Kit
Rapid method for the isolation and purification of total RNA and proteins sequentially from a single sample using the same column in less than 20 minutes
Fisher BioReagents™ SurePrep™ Nuclear or Cytoplasmic RNA Purification Kit
Rapid method for the isolation and purification of both nuclear and cytoplasmic RNA from cultured animal cells and animal tissue samples
Fisher BioReagents™ SurePrep™ RNA/DNA/Protein Purification Kit
Rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, tissue samples, blood, bacteria, yeast, fungi or plants
Fisher BioReagents™ SurePrep™ RNA Cleanup and Concentration Kit
Provides a rapid method to purify and concentrate RNA isolated using other methods such as phenol:chloroform extraction or after enzymatic reactions
MilliporeSigma™ Novagen™ T7 Tail Fiber Monoclonal Antibody
Used to immunoprecipitate T7 phage or T7 tail-fiber from solution.